AFM, CLSM and EIS characterization of the immobilization of antibodies on indium-tin oxide electrode and their capture of Legionella pneumophila.
Identifieur interne : 000250 ( Main/Exploration ); précédent : 000249; suivant : 000251AFM, CLSM and EIS characterization of the immobilization of antibodies on indium-tin oxide electrode and their capture of Legionella pneumophila.
Auteurs : RBID : pubmed:24274292Abstract
The microscopic surface molecular structures and properties of monoclonal anti-Legionella pneumophila antibodies on an indium-tin oxide (ITO) electrode surface were studied to elaborate an electrochemical immunosensor for Legionella pneumophila detection. A monoclonal anti-Legionella pneumophila antibody (MAb) has been immobilized onto an ITO electrode via covalent chemical bonds between antibodies amino-group and the ring of (3-Glycidoxypropyl) trimethoxysilane (GPTMS). The functionalization of the immunosensor was characterized by atomic force microscopy (AFM), water contact angle measurement, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in the presence of [Fe(CN)₆](3-/4-) as a redox probe. Specific binding of Legionella pneumophila sgp 1 cells onto the antibody-modified ITO electrode was shown by confocal laser scanning microscopy (CLSM) imaging and EIS. AFM images evidenced the dense and relatively homogeneous morphology on the ITO surface. The formation of the complex epoxysilane-antibodies acting as barriers for the electron transfer between the electrode surface and the redox species in the solution induced a significant increase in the charge transfer resistance (Rct) compared to all the electric elements. A linear relationship between the change in charge transfer resistance (ΔRct=Rct after immunoreactions - Rct control) and the logarithmic concentration value of L. pneumophila was observed in the range of 5 × 10(1)-5 × 10(4) CFU mL(-1) with a limit of detection 5 × 10(1)CFU mL(-1). The present study has demonstrated the successful deposition of an anti-L. pneumophila antibodies on an indium-tin oxide surface, opening its subsequent use as immuno-captor for the specific detection of L. pneumophila in environmental samples.
DOI: 10.1016/j.talanta.2013.09.049
PubMed: 24274292
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">AFM, CLSM and EIS characterization of the immobilization of antibodies on indium-tin oxide electrode and their capture of Legionella pneumophila.</title><author><name sortKey="Souiri, Mina" uniqKey="Souiri M">Mina Souiri</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory Advanced Materials and Interfaces, Faculty of Medicine, 5019 Monastir, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory Advanced Materials and Interfaces, Faculty of Medicine, 5019 Monastir</wicri:regionArea></affiliation></author><author><name sortKey="Blel, Nesrine" uniqKey="Blel N">Nesrine Blel</name></author><author><name sortKey="Sboui, Dejla" uniqKey="Sboui D">Dejla Sboui</name></author><author><name sortKey="Mhamdi, Lotfi" uniqKey="Mhamdi L">Lotfi Mhamdi</name></author><author><name sortKey="Epalle, Thibaut" uniqKey="Epalle T">Thibaut Epalle</name></author><author><name sortKey="Mzoughi, Ridha" uniqKey="Mzoughi R">Ridha Mzoughi</name></author><author><name sortKey="Riffard, Serge" uniqKey="Riffard S">Serge Riffard</name></author><author><name sortKey="Othmane, Ali" uniqKey="Othmane A">Ali Othmane</name></author></titleStmt><publicationStmt><date when="2014">2014</date><idno type="doi">10.1016/j.talanta.2013.09.049</idno><idno type="RBID">pubmed:24274292</idno><idno type="pmid">24274292</idno><idno type="wicri:Area/Main/Corpus">000275</idno><idno type="wicri:Area/Main/Curation">000275</idno><idno type="wicri:Area/Main/Exploration">000250</idno></publicationStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The microscopic surface molecular structures and properties of monoclonal anti-Legionella pneumophila antibodies on an indium-tin oxide (ITO) electrode surface were studied to elaborate an electrochemical immunosensor for Legionella pneumophila detection. A monoclonal anti-Legionella pneumophila antibody (MAb) has been immobilized onto an ITO electrode via covalent chemical bonds between antibodies amino-group and the ring of (3-Glycidoxypropyl) trimethoxysilane (GPTMS). The functionalization of the immunosensor was characterized by atomic force microscopy (AFM), water contact angle measurement, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in the presence of [Fe(CN)₆](3-/4-) as a redox probe. Specific binding of Legionella pneumophila sgp 1 cells onto the antibody-modified ITO electrode was shown by confocal laser scanning microscopy (CLSM) imaging and EIS. AFM images evidenced the dense and relatively homogeneous morphology on the ITO surface. The formation of the complex epoxysilane-antibodies acting as barriers for the electron transfer between the electrode surface and the redox species in the solution induced a significant increase in the charge transfer resistance (Rct) compared to all the electric elements. A linear relationship between the change in charge transfer resistance (ΔRct=Rct after immunoreactions - Rct control) and the logarithmic concentration value of L. pneumophila was observed in the range of 5 × 10(1)-5 × 10(4) CFU mL(-1) with a limit of detection 5 × 10(1)CFU mL(-1). The present study has demonstrated the successful deposition of an anti-L. pneumophila antibodies on an indium-tin oxide surface, opening its subsequent use as immuno-captor for the specific detection of L. pneumophila in environmental samples.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="In-Process"><PMID Version="1">24274292</PMID><DateCreated><Year>2013</Year><Month>11</Month><Day>26</Day></DateCreated><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-3573</ISSN><JournalIssue CitedMedium="Internet"><Volume>118</Volume><PubDate><Year>2014</Year><Month>Jan</Month><Day>15</Day></PubDate></JournalIssue><Title>Talanta</Title><ISOAbbreviation>Talanta</ISOAbbreviation></Journal><ArticleTitle>AFM, CLSM and EIS characterization of the immobilization of antibodies on indium-tin oxide electrode and their capture of Legionella pneumophila.</ArticleTitle><Pagination><MedlinePgn>224-30</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.talanta.2013.09.049</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0039-9140(13)00786-8</ELocationID><Abstract><AbstractText>The microscopic surface molecular structures and properties of monoclonal anti-Legionella pneumophila antibodies on an indium-tin oxide (ITO) electrode surface were studied to elaborate an electrochemical immunosensor for Legionella pneumophila detection. A monoclonal anti-Legionella pneumophila antibody (MAb) has been immobilized onto an ITO electrode via covalent chemical bonds between antibodies amino-group and the ring of (3-Glycidoxypropyl) trimethoxysilane (GPTMS). The functionalization of the immunosensor was characterized by atomic force microscopy (AFM), water contact angle measurement, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) in the presence of [Fe(CN)₆](3-/4-) as a redox probe. Specific binding of Legionella pneumophila sgp 1 cells onto the antibody-modified ITO electrode was shown by confocal laser scanning microscopy (CLSM) imaging and EIS. AFM images evidenced the dense and relatively homogeneous morphology on the ITO surface. The formation of the complex epoxysilane-antibodies acting as barriers for the electron transfer between the electrode surface and the redox species in the solution induced a significant increase in the charge transfer resistance (Rct) compared to all the electric elements. A linear relationship between the change in charge transfer resistance (ΔRct=Rct after immunoreactions - Rct control) and the logarithmic concentration value of L. pneumophila was observed in the range of 5 × 10(1)-5 × 10(4) CFU mL(-1) with a limit of detection 5 × 10(1)CFU mL(-1). The present study has demonstrated the successful deposition of an anti-L. pneumophila antibodies on an indium-tin oxide surface, opening its subsequent use as immuno-captor for the specific detection of L. pneumophila in environmental samples.</AbstractText><CopyrightInformation>© 2013 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Souiri</LastName><ForeName>Mina</ForeName><Initials>M</Initials><Affiliation>Laboratory Advanced Materials and Interfaces, Faculty of Medicine, 5019 Monastir, Tunisia.</Affiliation></Author><Author ValidYN="Y"><LastName>Blel</LastName><ForeName>Nesrine</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Sboui</LastName><ForeName>Dejla</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Mhamdi</LastName><ForeName>Lotfi</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Epalle</LastName><ForeName>Thibaut</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Mzoughi</LastName><ForeName>Ridha</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Riffard</LastName><ForeName>Serge</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Othmane</LastName><ForeName>Ali</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType>Journal Article</PublicationType><PublicationType>Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>10</Month><Day>15</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Talanta</MedlineTA><NlmUniqueID>2984816R</NlmUniqueID><ISSNLinking>0039-9140</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Antibody immobilization</Keyword><Keyword MajorTopicYN="N">Detection</Keyword><Keyword MajorTopicYN="N">Electrochemical impedance spectroscopy</Keyword><Keyword MajorTopicYN="N">Immunosensor</Keyword><Keyword MajorTopicYN="N">Legionella pneumophila</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>5</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>9</Month><Day>24</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>9</Month><Day>26</Day></PubMedPubDate><PubMedPubDate PubStatus="aheadofprint"><Year>2013</Year><Month>10</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>11</Month><Day>27</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>11</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2013</Year><Month>11</Month><Day>28</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pii">S0039-9140(13)00786-8</ArticleId><ArticleId IdType="doi">10.1016/j.talanta.2013.09.049</ArticleId><ArticleId IdType="pubmed">24274292</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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